Cultural, Morphological and Molecular Characterization of Vinca Alkaloids Producing Endophytic Fungus Fusarium Solani Isolated from Catharanthus Roseus
نویسندگان
چکیده
We have isolated fifty-two endophytic fungi (AA-CRL-1 to AA-CRL52) from the leaves of Catharanthus roseus plants collected from different parts of Pune (Maharashtra, India). These endophytic fungi represented different genera and were screened for vinca alkaloids production using TLC and ESI-MS analysis. Out of the 52 endophytic fungal cultures screened, the endophytic culture AA-CRL-20 was found to produce vinca alkaloids (Vinblastine and Vincristine) extracellularly. The endophytic fungal strain AA-CRL-20 which produced vinca alkaloids was identified as Fusarium solani based on its cultural and morphological characters and internal transcribed spacer (ITS) sequence analysis.
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